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KMID : 0350519930460020647
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Journal of Catholic Medical College
1993 Volume.46 No. 2 p.647 ~ p.655
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Human Umbilical Cord Blood as a Potential Source of Transplantble Hematopoietic Stem Cells
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Abstract
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Bone marrow transplantation is currently the treatment of choice for selected patients with aplastic anemia, leukemia, and inherited disorders of the bone marrow. The reconstitution of hematopoiesis requires the transplantation of hematopoietic
stem
cells(HSCs), which are recovered from the bone marrow of related or unrelated donors. In the practical point of view, suitable marrow is frequently not available. Human umbilical cord blood may be one of the potential sourse of HSC.
This study was performed to evaluate human umbilical cord blood as an alternative to bone marrow in the provision of transplantable HSCs for hematopoietic reconstitution. We examined 21 collections of hyman umbilical cord blood for their content
of
total nucleated cells and mononuclear cells, colony count of CFU-GM and CFU-GEMM before and after cryopreservation using hyman placental conditioned medium and phytohemagglutinin-lymphocyte conditioned medium and investigated the distribution of
CD34
positive cells reactive with anti-HPCA-1 antibody determined by indirect immunofluorescence staining and flow cytometry.
@ES The results were as follows ;
@EN 1) The numbers of total nucleated cells and mononuclear cells in human umbilical cord blood were significantly higher than those in normal bone marrow.
2) No significant difference of the number of CFU-GM colonies after 7 days culture was observed between human umbilical cord blood and normal bone marrow. However, the numbers of CFU-GM colonies after 14 days culture and CFU-GEMM colonies after
10
days
culture were significantly higher in human umbilical cord blood than in normal bone marrow.
3) The percentage of CD34 positive cells was tended to be higher in human umbilical cord blood than in normal bone marrow.
4) The numbers of CFU-GM and CFU-GEMM colonises showed no significant diffcrences after cryopreservation.
These results suggest that the human umbilical cord blood is sufficient to serve as a potential source of transplantable HSCs. In the future, it may be possible to use a human umbilical cord blood bank in order to solve the logistic problems of
unrelated donor searches.
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